Akademik Veri Yönetim Sistemi
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, cilt.12, sa.6, ss.726-730, 2013 (SCI-Expanded)
The response to superovulatory treatment and the embryo yield of Dorper ewes in a non-breeding season were evaluated. Average 65 kg, weight, 3 years old donor ewes were inserted CIDR for 12 days and divided into four superovulation treatment groups; Exp. I: 12 mL Folltropin+200 I.U. eCG (n = 25); Exp. II: 10 mL Folltropin+200 I.U. eCG (n = 20); Exp. III: 10 mL Folltropin+600 I.U. eCG (n = 25) and Exp. IV: 10 mL Folltropin+750 I.U. eCG (n = 10) with single shot of FSH at CIDR removal. Donors were superovulated using FSH-p (total of 20 mg) (Folltropin-V; Vetrepharm, Canada) applied in six decreasing doses of 3, 2.5, 2, 2, 1.5, 1 mL i.m. starting 60 h before CIDR withdrawal. Donors underwent intrauterine All with fresh diluted semen (a minimum of 50x10 motile sperm/each uterine horn) 40 h after CIDR removal and embryos collected 6 days after insemination. The CL responses were found to be similar in all treatment groups (Exp. I: 11.7+1.18; II: 9.7+1.31; III: 8.4+1.76 and IV: 9.9+1.63). Superovulation response CL>3) was found relatively but not significantly higher in lower dose of eCG treated Exp. I: 92% and II: 91%, respectively compared to III: 76% and IV: 75%. Transferable embryo numbers for a fresh MOET program were found slightly higher (P: 0.333) in donors in Exp. III with 7.8 +1.2 than those recorded for donors in Exp. I (4.7+1.12), II (6.3+1.24) and IV (6.3+1.7). The significant difference (p<0.05) was observed in embryo recovery rates; Exp. II and III: 63%, Exp. I: 38% and IV: 43%. Stages of embryonic development were also affected by the different hormonal treatments used in the study Exp. I and IV giving the best results for a fresh MOET program. In conclusion, it was found that Dorper ewes treated with different superovulation protocols in out of season (May) in vivo fresh embryo MOET program yielded 9 embryos and the recovery rate was markedly higher than those of earlier findings. Study also showed that a single FSH injection can be used in place of decreasing dose of multiple FSH injections with similar results.