In vitro effects of some drugs on catalase purified from human skin

ALTIKAT S., COBAN A., Ciftci M., Ozdemir H.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.21, sa.2, ss.231-234, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 2
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/14756360500483453
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.231-234
  • Anahtar Kelimeler: catalase, drug, purification, human skin, inhibition, ENZYME-ACTIVITY, PURIFICATION, GLUCOSE-6-PHOSPHATE-DEHYDROGENASE, ERYTHROCYTES, STRESS, VIVO
  • Atatürk Üniversitesi Adresli: Evet

Özet

Catalase enzyme (H2O2: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4 degrees C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I-50 values of 0.414 1.29, 1.8, 3.83, and 8.64 mM, respectively.