Investigating effects of locally applied boric acid on fracture healing with and without low-level laser therapy

TURGUT F., Yanmaz L. E.

Lasers in Medical Science, cilt.38, sa.1, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10103-022-03695-w
  • Dergi Adı: Lasers in Medical Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CINAHL, Communication Abstracts, EMBASE, MEDLINE
  • Anahtar Kelimeler: BMP-2, Borax, Boron, Osteoblast, Photobiomodulation, Photobiostimulation
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2022, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.This study aimed to investigate the effects on fracture healing of locally applied boric acid (BA) with and without low-level laser therapy (LLLT). A unicortical femoral defect was surgically created on the anterolateral surface of proximal femur of each subject. The subjects, totaling 56 Wistar albino rats, were randomly allocated into four groups (n = 14 each): control, LLLT (λ = 905 μm, 10,000 Hz, 25 mW, and peak power 25 W), BA (40 mg/kg), and BA + LLLT groups. On the 30th day, the highest radiological score was recorded for the BA + LLLT group (3.63 [2–4]), followed by the BA (3.38 [2.75–3.75]), control (3 [2–3.25]), and LLLT (2.5 [1.25–3]) groups. On days 15 and 30 post-surgery, malondialdehyde levels were significantly lower among the BA + LLLT group compared to the control group (p < 0.001). On day 30, superoxide dismutase, catalase, and alkaline phosphatase levels were highest in the BA + LLLT group compared to the control group (p < 0.001). When the histopathological, immunofluorescence, and immunohistochemical findings on the 15th and 30th days were compared with the control group, a statistically significant difference was found for the BA and BA + LLLT groups (p ˂ 0.05). This study suggests that locally applied BA with LLLT may accelerate fracture healing.