Akademik Veri Yönetim Sistemi
II. İnternational VET istanbul Group Congress, Sankt-Peterburg, Rusya, 7 - 09 Nisan 2015, ss.28
lntroduction: Gentamicin (G) is an aminoglycoslde antibiotic that has a broad spec..
trum agalnst microbial pathogens, especially gram-negative bacteria. However, it is well
known that gentamicin has some adverse effects such ototoxicity and testicular toxicity.
Taraxacum officinale extract (TOE) has been widely used as a folkloric medicine for the
treatment of diverse diseases. Furthermore, TOE has inhibiting effect on reactive oxygen
species. The aim of this study was to investigation of the effects of TOE on gentamycin
induced reproductive damage in male rats.
Material and methods: in the study, 24 male Sprague Dawley rats aged eight weeks
old and weighted 250-300 gr, were used in this study. The animals were obtained from
Atatürk University Experimental Research Centre and housed in standard laboratory conditions.
Commercial pellet chow and fresh drinking water were available ad libitum. Rats
were divided into four groups. 1. group (n= 6) referred as control group and physiological
saline via oral gavage to the animals il. group (n=6) referred as Gentamicin (G) and
2-mg;kg gentamicin sulphate (GS) was injected intraperitonally (iP) to animals. 111. Group
(n=6) was referred as G + TOE150, 2-mglkg GS and 150 mglkg TOE injected iP to animals.
iV. group was referred as G+ TOE200 (n=6), 2-mglkg GS and 200 mg/kg TOE injected
iP to anlmals. The treatment was performed fer consecutive 7 days. The animals were
tranquilized and sacriflced on sııı day of study. Cauda epididymal semen samples and
testes tissues were collected. Routine semen examinations were performed and oxidative
stress levels of testicular tissues were assayed. Reproductive organ weights (total testes
weight (TlW) and total cauda epididymides weights (TCEW) ] were recorded. Velocity
of sperm cells in G, G+TOE150 and G+TOE200 groups were signiflcantly lower when
compared to control group. On the other hand, motility in G group was significantly lower
when compared to control group. However, !here were no difference between in treatments
G+TOE150, G+TOE200 groups. Furhermore, there were ne differences in terms
of dead sperm rate, sperm density, total abnormality, TIW and TCEW among ali groups.
MDA level of G group was significantly higher than in control and treatment groups.
Glutathione peroxidase (GPx) and glutathione (GSH) levets in G group were lower than
the other groups. The lowest SOD level was in G group while the highest level was in
control group. MDA level was higher and CAT level was lower in G group when compared
to other groups.
Conclusion: The dose of G +150mg/kg and G +200 mg/kg TOE is effective on mentioned
spermatological parameters induced by gentamicin.
Kay words: Taraxacum offıcinale, gentamycin, reproduction, rat.