Isolation and Identification of Pathogenic Bacteria in the Digestive Microbiota of Aquatic Beetles

Bektaş M., Orhan F., Barış Ö.

ARCHIVES OF MICROBIOLOGY, cilt.204, sa.12, ss.1-10, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 204 Sayı: 12
  • Basım Tarihi: 2022
  • Dergi Adı: ARCHIVES OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1-10
  • Atatürk Üniversitesi Adresli: Evet

Özet

Insects, leading the richest group in terms of species diversity in the world of living organisms, display the largest diversity in terms of feeding, habitat and adaptation to different living environments. In addition to studies related to the use of insects in the search for alternative foods in the world in general, the focus has been on toxicologic and pathogenic research about insects. The habitats of aquatic beetles are basically the water sources used for both agricultural and drinking water, so aquatic beetles gain further importance. Aquatic insects from the families Helophoridae and Hydrophiliae were collected from different localities in Erzurum and surroundings. After 17 beetle’s species were identified and its digestive tracts were dissected under aseptic conditions and microbiological analyses were performed. Isolates were identified by analyzing molecular and conventional data together. In addition to conventional analyses, 16S rDNA gene sequences were amplified with PCR reaction and DNA sequencing analyses were performed. After BLAST analysis of the obtained DNA sequences, GenBank accession numbers were obtained. In this study, the aim was to identify definite and potential human pathogens in bacteria isolated from the intestinal microbiota cultures, characterized with conventional and molecular methods, of insects among the aquatic beetles.