Akademik Veri Yönetim Sistemi
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.22, sa.5, ss.647-651, 2016 (SCI-Expanded)
The most crucial problem in experimentally induced excisional wounds in animals with slim-skinned and poor subcutaneous attachment is wound contraction formed during recovery period. The contraction is mainly induced by myofibroblasts leading to early wound closure. This may be associated with false outcomes of the study. Recently, various trials have been made to reduce the contraction in full-thickness excisional models of wounds. This study aimed at comparing different fixation methods on the minimization of wound contraction. Study was carried out in two main groups as non-diabetic (Group I, n=24) and diabetic (Group II, n=24) and one control group. The animals in each group were divided into four equal subgroups (Group I-A, I-B, I-C, I-D; Group II-A, II-B, II-C, II-D) and 1.2x1.2 cm-sized full-thickness of skin excisions were generated on the dorsum of animals. No fixations were applied in subgroups I-A and II-A however, interrupted suture in subgroups I-B and II-B, suture passing from a couple of points through skin and subcutaneous tissues placed in each side of wound tied only in corners in subgroups I-C and II-C and using a polyethylene fixatives that fixed in wound edges with interrupted sutures in subgroups I-D and II-D were achieved to produce wound fixation methods. Digital photographs of the wound were taken on the 3rd, 7th, 14th and 21st days of the study, reduction ratios of the wounds were compared and the efficiency of methods for the prevention of contraction was examined. When wounds were evaluated comparatively after fixation, it was observed that three different applied fixation methods were efficient in prevention of wound contraction in all phases in comparison to control groups. Moreover, it was observed that contraction was highly impeded in interrupted suture groups (I-B, II-B) and polyethylene-reinforced interrupted suture groups (I-D, II-D) when compared to suture groups tied only in corners. The results revealed that wound surface area shrinkage should be taken into consideration in experimental studies to avoid misleading consequences.