Evaluation of Sheep Wool Protein Hydrolysate and Molasses as Low-Cost Fermentation Substrates for Hyaluronic Acid Production by Streptococcus zooepidemicus ATCC 35246

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Arslan N. P., Aydoğan M. N.

WASTE AND BIOMASS VALORIZATION, cilt.12, sa.2, ss.925-935, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s12649-020-01062-w
  • Dergi Adı: WASTE AND BIOMASS VALORIZATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Compendex, INSPEC, Veterinary Science Database
  • Sayfa Sayıları: ss.925-935
  • Anahtar Kelimeler: Streptococcus zooepidemicus, Sheep wool peptone, Molasses, Hyaluronic acid, AGROINDUSTRIAL BY-PRODUCTS, MICROBIAL-PRODUCTION, CULTURE, KERATIN, OPTIMIZATION, CULTIVATION, PEPTONE, SURFACE, WASTES, BATCH
  • Atatürk Üniversitesi Adresli: Evet

Özet

Peptones are widely used as complex fermentation substrate for hyaluronic acid (HA) production; however, use of peptones in HA production reduces commercial competitiveness due to their high price. The present study was conducted to test the feasibility of sheep wool peptone (SWP) (mainly organic nitrogen source) and molasses (mainly carbon source) as cheap substrates for HA production from Streptococcus zooepidemicus ATCC 35246. Six peptones (SWP I-VI) were prepared from sheep wool using different chemical hydrolysis methods. Among them, SWP-VI was determined to be more fovarable for HA production. SWP-VI was compared with commercial tryptone peptone (TP) and protease peptone (PP) in order to evaluate its effectiveness in production of HA, lactic acid (LA) and cell biomass (CB). The protein contents of SWP-VI, TP and PP were determined as 70.6, 83.1 and 83.8 g/100 g, respectively. The best peptone for HA and CB production was SWP-VI, whereas PP was found to be more favourable for LA production. Maximum HA concentrations in SWP-VI, TP and PP media were determined as 3.54, 2.58 and 2.47 g/L, respectively.