Akademik Veri Yönetim Sistemi
International Black Sea Modern Scientific Research Congress, Rize, Türkiye, 29 Eylül - 02 Ekim 2022
Nanobiotechnology, which deals with
nanoparticles (NPs), has been one of the most popular topics in recent years. NPs
are small materials ranging between 1 to 100 nanometers (nm) in size. They have
higher surface reactivity than their bulk materials and therefore, have
different properties and application areas. Although NPs
can also be synthesized by physical and chemical means, their biological
synthesis is preferred due to their toxicity and incompatibility issues. Green
synthesis of nanoparticles by bacteria offers a simple, cheap, and ecologically
friendly ways.
Antibiotic resistance
of pathogenic bacteria is a serious problem worldwide and novel
and potent bactericidal agents is of great importance.
In this study, silver nanoparticles (Ag-NPs) were synthesized by Pseudomonas aeruginosa
strain OG1 strain in nutrient broth medium containing 1 mM AgNO3.
They were analyzed by SEM, TEM and XRD and were found to be 30 nm in size.
Ag-NPs were used
for their antibacterial, antibiofilm and antiurease activities. Growth
inhibition zones of 0,8 mm and 1.1 mm with 100 µg/mL and 200 µg/mL Ag-NPs,
respectively, were obtained against Streptococcus
salivarius. While 100 and 200 µg/mL Ag-NP caused complete urease inhibition
(100%) in S. salivarius in the first
5 hours and 24 h incubation. 86 % and 90% inhibitions in biofilm formation were
observed with 100 µg/mL and 200 µg/mL Ag-NP, respectively. As a result, this
study showed that Ag-NPs can be used as effective antibacterial, antibiofilm
and antiurease agents against microorganisms that may be harmful to humans.