Evaluation of wool protein hydrolysate as peptone for production of microbial enzymes

Ömeroğlu M. A., Albayrak Ş., Arslan N. P., Özkan H., Adıgüzel A., Taşkın M.

3 Biotech, cilt.13, sa.1, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s13205-022-03456-0
  • Dergi Adı: 3 Biotech
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Bacillus licheniformis, Aspergillus niger, Industrial enzymes, Keratinous proteins, Sheep wool protein hydrolysate, SOLID-STATE FERMENTATION, WASTE CHICKEN FEATHERS, CITRIC-ACID PRODUCTION, SUBMERGED FERMENTATION, PARTIAL-PURIFICATION, CULTURE-CONDITIONS, LIPASE PRODUCTION, INVERTASE, CELLULASE, TANNASE
  • Atatürk Üniversitesi Adresli: Evet

Özet

© 2023, King Abdulaziz City for Science and Technology.Peptones are one of the most expensive components of microbial culture media. The present study was conducted to test the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes (lipase, amylase, tannase, pectinase, cellulase and invertase). SWP was prepared by alkaline hydrolysis and acid neutralization. Bacillus licheniformis and Aspergillus niger were selected as test microorganisms for enzyme production. To evaluate the efficacy of SWP in enzyme production, it was compared with commercial tryptone peptone (TP) in the shaking flask cultures of the test microorganisms. The optimum concentration of both SWP and TP was determined to be 8 g/L for the production of B. licheniformis-derived enzymes, but 6 g/L for the production of A. niger-derived enzymes. It was determined that SWP was superior to TP in the production of four enzymes (lipase, amylase, tannase and pectinase) of both B. licheniformis and A. niger. This is the first study about the usage of sheep wool protein hydrolysate (SWP) as an organic nitrogen source or a peptone in fermentative production of microbial enzymes.