Akademik Veri Yönetim Sistemi
10. Ulusal 3. Uluslararası Hidatidoloji Kongresi, İzmir, Türkiye, 4 - 06 Şubat 2022, ss.139
Objectives: With this study, it was aimed to
investigate the presence of Echinococcus
species in a wolf.
Materials-Methods: The necropsy of the wolf which
was found dead in Erzurum's Yakutiye district was
carried out. The intestinal contents were examined
under a stereomicroscope with the sedimentation
and counting technique. Adult parasites were evaluated according to morphological criteria. Isolation
of DNA from adult parasites was executed. Echinococcus granulosus adults were subjected to Polymerase Chain Reaction (PCR) with JB3-JB4.5 primer pairs which amplify the cytochrome c oxidase subunit
1 (COI) gene. Echinococcus multilocularis adults
were subjected to PCR with Emnest/for and Emnest/rev primer pairs which amplify the 12S rRNA
gene region. The obtained amplicons were sent for
sequence analysis. The sequence data were edited,
aligned, and compared with reference isolates.
Then nucleotide sequences of E.multilocularis and
E.granulosus sensu stricto (s.s.) isolates were registered into the GenBank database.
Results: Echinococcus spp. were detected by sedimentation and counting technique. Species were
identified as E.granulosus and E.multilocularis according to morphological criteria. The result of
BLAST analysis of the adult E.granulosus sequences
was identified as G1 genotype (Accession no;
OK357541). Echinococcus multilocularis was confirmed by the sequence analysis of the obtained PCR
amplicons (Accession no; MT321279).
Conclusions: The study is presented the first report
of the presence of E.granulosus s.s and E.multilocularis in a wolf in Turkey. The detection of both adult
parasites in the same final host confirms that the
study result is supported that Erzurum is an endemic region for cystic and alveolar echinococcosis
thanks to presence of both Echinocccus adults in
one definitive host.