Effects of antiemetic drugs on glucose 6-phosphate dehydrogenase and some antioxidant enzymes

Ozmen I., Kufrevioglu Ö. İ.

PHARMACOLOGICAL RESEARCH, cilt.50, sa.5, ss.499-504, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 5
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.phrs.2004.05.007
  • Dergi Adı: PHARMACOLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.499-504
  • Anahtar Kelimeler: glucose-6-phosphate dehydrogenase, antioxidant enzymes, purification, inhibition, antiemetic drugs, ETHANOL, BRAIN, PURIFICATION, ERYTHROCYTE, PROTECTION, MECHANISMS, EXERCISE, CLEAVAGE, CATALASE, STRESS
  • Atatürk Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate effect of antiemetics on G6PD and antioxidant enzymes. Antiemetics are currently being used to reduce or prevent nausea and vomiting in patients. This is the first study to show effect of antiemetics on glucose-6-phosphate dehydrogenase (G6PD) and antioxidant enzyme activities. For in vitro studies, G6PD was purified from human erythrocyte, 10, 26-fold in a yield of 51.3% by using ammonium sulphate precipitation and 2',5'-ADP-Sepharose 413 affinity gel. The purified enzyme showed a single band on sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS-PAGE). The effects of four different antiemetics (granisetron hydrochloride, ondansetron hydrochloride, metoclopramide hydrochloride, trimethobenzamide hydrochloride) were investigated on the purified enzyme. Granisetron hydrochloride and ondansetron hydrochloride inhibited the enzyme activity (K-i values; 5.05 mM and 0.034 mM, I-50 values; 3.9 mM and 0.036 mM, respectively). Metoclopramide hydrochloride, trimethobenzamide hydrochloride showed no inhibition effects.