Seroprevalence of Hypodermosis in Cattle in Nigde Province of Turkey by Comparison of Commercial and Indirect-ELISA Methods

KARATEPE M., ŞİMŞEK S., KARATEPE B., CAYVAZ M., SEVGİLİ M., BALKAYA İ.

ISRAEL JOURNAL OF VETERINARY MEDICINE, cilt.68, sa.1, ss.38-42, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 1
  • Basım Tarihi: 2013
  • Dergi Adı: ISRAEL JOURNAL OF VETERINARY MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.38-42
  • Anahtar Kelimeler: Hypoderma spp., hypodermosis, Enzyme-Linked Immunosorbent Assay (ELISA), seroprevalence, cattle, Nigde, Turkey, FRAGMENT-LENGTH-POLYMORPHISM, POLYMERASE-CHAIN-REACTION, BOVINE HYPODERMOSIS, LINEATUM DIPTERA, OESTRIDAE, DIFFERENTIATION, SURVIVAL
  • Atatürk Üniversitesi Adresli: Evet

Özet

The aim of this study was to determine the seroprevalence of hypodermosis in cattle of Nigde province, located in the centre of Turkey. A total of 183 serum samples were collected between December 2009 and February 2010 for this goal. The serum samples were analyzed with a commercial ELISA kit (Pourquier ELISA Hypodermosis bovine serum screening) and an indirect-ELISA (using antigen Hypodermin C) methods. The results of the tests with the commercial ELISA kit showed that 53 (28.9%) out of the 183 cattle were seropositive for hypodermosis. Eleven (18.3%) out of the 60 female and 42 (34.1%) out of the 123 male, were found to be seropositive. The seropositivity rate was calculated as 21.8% (40/183) by indirect-ELISA, and the seroprevalence was 18.3% (11/60) and 23.5% (29/123) in female and males, respectively. A statistically significant correlation was observed when the two test methods for detecting seropositivity of Hypoderma were compared (p<0.001). This study is to the best of our knowledge the first serological survey on hypodermosis in cattle from the Nigde province of Turkey.