Rutin Attenuates Gentamicin Induced Renal Damage By Reducing The Oxidative Stress Inflammation Apoptosis And Autophagy In Rats

Kandemir F. M., Özkaraca M., Apaydın Yıldırım B., Hanedan B., Aktaş Şenocak E., Benzer F.

International Scientific Congress, Scientific Papers Veterinary Medicine, Iasi, Romanya, 23 - 25 Ekim 2014, ss.43

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Iasi
  • Basıldığı Ülke: Romanya
  • Sayfa Sayıları: ss.43
  • Atatürk Üniversitesi Adresli: Evet

Özet

Abstract

The aim of this study was to evaluate the effect of rutin on oxidative stress, inflammation, apoptosis, and

autophagy in gentamicin-induced nephrotoxicity in rats. The administration of gentamicin at a dose of 80

mg/kg/d for 8 days intraperitoneally induced nephrotoxicity as evidenced by increased serum urea and creatinine

levels, and histopathology. Rutin treatment 6 days before gentamicin administration and co-treatment with

gentamicin for 8 days in gentamicin + rutin group attenuated nephrotoxicity induced by gentamicin by reducing

serum urea, creatinine, malondialdehyde (MDA), increasing superoxide dismutase (SOD), catalase (CAT),

glutathione peroxidase (GPx) activities and reduced glutathione (GSH) content, and inhibiting the inducible

nitric oxide synthase (iNOS), cleaved caspase 3, and light chain 3B (LC3B) expression as evidenced by

immunohistochemical staining. The present study demonstrated that rutin has anti-oxidant, anti-inflammatory,

antiapoptotic, antiautophagic effects, and that rutin treatment could attenuate gentamicin-induced nephrotoxicity.

Keywords: Gentamicin, Rutin, Nephrotoxicity, Oxidative stress, Apoptosis, Autophagy.