Simple Sequence Repeat and S-locus Genotyping to Explore Genetic Variability in Polyploid Prunus spinosa and P. insititia


HALASZ J., MAKOVICS-ZSOHAR N., SZOKE F., ERCİŞLİ S., HEGEDUS A.

BIOCHEMICAL GENETICS, cilt.55, sa.1, ss.22-33, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 1
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s10528-016-9768-3
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.22-33
  • Anahtar Kelimeler: Prunus spinosa, Prunus insititia, SSR, S-genotype, Polyploid, MICROSATELLITE MARKERS, APPLE CULTIVARS, PCR DETECTION, INCOMPATIBILITY, ROSACEAE, ALLELES, L., PRIMERS, ALMOND, POPULATIONS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Polyploid Prunus spinosa (2n = 4x) and P. insititia (2n = 6x) represent enormous genetic potential in Central Europe, which can be exploited in breeding programmes. In Hungary, 17 cultivar candidates were selected from wild-growing populations including 10 P. spinosa, 4 P. insititia and three P. spinosa 9 P. domestica hybrids (2n = 5x). Their taxonomic classification was based on their phenotypic characteristics. Six simple sequence repeats (SSRs) and the multiallelic S-locus genotyping were used to characterize genetic variability and reliable identification of the tested accessions. A total of 98 SSR alleles were identified, which presents 19.5 average allele number per locus, and each of the 17 genotypes could be discriminated based on unique SSR fingerprints. A total of 23 S-RNase alleles were identified. The complete and partial S-genotype was determined for 8 and 9 accessions, respectively. The identification of a cross-incompatible pair of cultivar candidates and several semi-compatible combinations help maximize fruit set in commercial orchards. Our results indicate that the S-allele pools of wild-growing P. spinosa and P. insititia are overlapping in Hungary. A phylogenetic and principal component analysis confirmed the high level of diversity and genetic differentiation present within the analysed genotypes and helped clarify doubtful taxonomic identities. Our data confirm that S-locus genotyping is suitable for diversity studies in polyploid Prunus species. The analysed accessions represent huge genetic potential that can be exploited in commercial cultivation.