Determination of 17 beta-Estradiol in Rabbit Plasma by Gas Chromatography with Flame Ionization Detection

YILMAZ B., KADIOĞLU Y.

INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.74, sa.3, ss.248-253, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 74 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.4103/0250-474x.106067
  • Dergi Adı: INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.248-253
  • Anahtar Kelimeler: Flame ionization detection, gas chromatography, liquid-liquid extraction, pharmacokinetics, validation, 17 beta-estradiol, PERFORMANCE LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, DEGRADATION-PRODUCTS, 17-BETA-ESTRADIOL, WATER, DERIVATIZATION, VALIDATION, ESTROGENS, HORMONES, URINE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Yilmaz and Kadioglu: Determination of 17 beta-Estradiol in Rabbit Plasma This article describes gas chromatography-flame ionization detection method for determination of 17 beta-estradiol in rabbit plasma. 17 beta-estradiol and internal standard progesterone were extracted from plasma using liquid-liquid extraction method. Linearity was found between 0.25 and 20 mu g/ml (r(2)=0.994) for plasma samples. Intra-day and inter-day precision, expressed as the relative standard deviation were less than 5.5%, and accuracy (relative error) was less than 3.5%. The mean recovery of 17 beta-estradiol samples was 94.4%. The limits of detection and quantification of method for plasma samples were 0.10 mu g/ml and 0.15 mu g/ml, respectively. Also, clinically used other 10 drugs were investigated to check for potential interferences and the method was successfully applied to the determination of 17 beta-estradiol in New Zealand white rabbits.