Assessment of population structure, genetic diversity and relationship of Mediterranean olive accessions using SSR markers and computational tools

Ben Ayed R., ERCİŞLİ S., Hanana M., Rebai A., Moreau F.

BIOTECHNOLOGY LETTERS, cilt.44, sa.1, ss.113-127, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10529-021-03204-z
  • Dergi Adı: BIOTECHNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.113-127
  • Anahtar Kelimeler: Olea europaea L, Mediterranean basin, UPGMA, PCoA, Population structure, Markov Chain Monte Carlo (MCMC), Bayesian statistics, OLEA-EUROPAEA L., SEQUENCE REPEATS SSRS, MICROSATELLITE MARKERS, MOROCCAN OLIVE, DNA, DOMESTICATION, CULTIVARS, TREE, OIL, IDENTIFICATION
  • Atatürk Üniversitesi Adresli: Evet

Özet

Olive tree is an emblematic crop of the Mediterranean region, mainly renowned for its fruit oil, although the species provides several industrial purposes. The Mediterranean basin constitutes the origin of olive species diversification and represents a valuable source of genetic variability of olive germplasm. Therefore, the evaluation of the diversity and the population structure of this Mediterranean germplasm is a challenge for olive species preservation, crop breeding and genetic improvement. In this context, our study aims to analyze the genetic diversity and the population structure of 79 Mediterranean olive accessions using 15 genomic SSRs and by applying computational model-based approaches. The used SSRs revealed a total number of 225 alleles with a mean of 15 alleles per locus. Observed and expected heterozygosity (Ho = 0.79, He = 0.805) with a Polymorphism Information Content value of 0.775 indicate high level of genetic diversity. All results of the Unweighted Pair Group Method with Arithmetic (UPGMA), Jaccard similarity index, Principal Coordinate Analysis (PCoA) and the Bayesian analyses supported the separation of the Mediterranean varieties in two sub-populations, one of which mainly composed by Spanish accessions.