A new update for radiocontrast-induced nephropathy aggravated with glycerol in rats: the protective potential of epigallocatechin-3-gallate


Palabiyik S. S. , DINCER B., ÇADIRCI E. , CINAR I., Gundogdu C., Polat B. , ...More

RENAL FAILURE, vol.39, no.1, pp.314-322, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 1
  • Publication Date: 2017
  • Doi Number: 10.1080/0886022x.2016.1277245
  • Title of Journal : RENAL FAILURE
  • Page Numbers: pp.314-322

Abstract

Contrast media (CM) is known to have nephrotoxic adverse effects. Epigallocatechin-3-gallate (EGCG) is the most abundant and active catechin in green tea, and has strong antioxidant and anti-inflammatory properties. This study investigated whether EGCG can reduce contrast-induced nephrotoxicity (CIN), alone or with glycerol (GLY)-induced renal damage, and to understand its mechanisms of protection against toxicity, using models of GLY and CIN in rats. The rats were separated into eight groups (n = 6 in each), as follows: Healthy, GLY, CM, GLY+CM, CM+EGCG 50 mg/kg (po), GLY+CM+EGCG 50 mg/kg (po), CM+EGCG 100 mg/kg (po), and GLY+CM+EGCG 100 mg/kg (po). Both doses of EGCG protected against CM-induced renal dysfunction, as measured by serum creatinine and blood urea nitrogen (BUN). In addition, EGCG treatment markedly improved CIN-induced oxidative stress, and resulted in a significant downregulatory effect on tumor necrosis factor (TNF)-alpha and nuclear factor (NF)-kappa B mRNA expression. Moreover, histopathological analysis showed that EGCG also attenuated CM-induced kidney damage. Considering the potential clinical use of CM and the numerous health benefits of EGCG, this study showed the protective role of multi-dose EGCG treatment on CIN and GLY-aggravated CIN through different mechanisms.