Research Information System
Thesis Type: Postgraduate
Institution Of The Thesis: Ataturk University, Sağlık Bilimleri Enstitüsü, Turkey
Approval Date: 2020
Thesis Language: Turkish
Student: EYLÜL TURUNÇ
Supervisor: Serkan Yıldırım
Abstract:
Aim: Cisplatin (CP) is an antineoplastic or cytotoxic chemotherapy drug.
Recently, the number of studies using herbal compounds to increase the number of
cytotoxic effects of antineoplastic drugs is increasing. One of the compounds used for
this purpose is Lactobacillus lactis probiotic. In this study, it was aimed to investigate the
protective effects of Lactobacillus lactis on histopathology of the heart and some enzymes
and apoptotic markers of CP-induced cardiotoxicity.
Material and Methods: In this study, 28 male Sprague Dawley rats weighing
approximately 200-250 g were used. In order to provide sufficient tissue samples in each
group, 7 rats were used in each group and 4 groups were formed.
All animals were subjected to standard maintenance and feeding conditions.
Group 1 received 1 ml distilled water intragastric (i.g.) for 7 days, Group 2 was given
distilled water (i.g) for 4 days and in the experiment between 5-7 days cisplatin (7.5 mg /
kg) was administered intraperitoneally (i.p.). Group 3 were applied probiotics 1 ml / mg
/ kg (i.g.) for 7 days. Group 4 were applied Lactobacillus lactis probiotics 1 ml / mg / kg
doses (i.g) for 7 days. On the 5th day of the experiment, i.p. Cisplatin (7.5 mg / kg dose)
was injected. At the end of the experimental applications, blood and heart tissues were
collected. Biochemically, MDA, SOD, GSH, NO, Troponin I, CK, CK-MB and LDH
levels were determined. Cardiac tissue routine histopathology was performed and Tnf α,
Caspase-3, and Bcl-2 staining were performed for immunohistochemistry and examined
under the light microscope.
Results: CP was found to have a cardiotoxic effect on the histological structure of
the heart, some enzymes, antioxidants and immunohistochemistry in rats. Lactobacillus
lactis, used as a probiotic, was found to suppress this cardiotoxic effect.
Conclusion: It has been shown that Lactobacillus lactis has biochemical,
histopathological and immunohistochemically protective effects on cardiotoxicity in CPinduced rats.