Akademik Veri Yönetim Sistemi
TÜBİTAK Projesi, 2022 - 2023
Organ and tissue loss remain significant challenges for human health, and transplantation from deceased or living donors is currently the primary treatment method. However, advances in technology have led to studies suggesting that in the near future, patient-derived cells could be used to create personalized organs and tissues. While cell-cell interaction is an essential consideration for such advancements, the interaction between cells and materials also plays a vital role. In particular, specific cell lineages, such as heart muscles, respond to electrical stimulation, which requires additional engineering intervention, including the use of scaffolds and devices made from materials such as polymers and metals (Clegg et al., 2019; Parihar et al., 2022). The most advanced method used in today's technology for the production of scaffolds is 3D bio-printing, which is a promising technology for creating living tissue using cellincluded bioinks or biological materials that support cell viability. Fibers produced with the melt electrowriting (MEW) technique, in particular, are advantageous due to their micro and nano sizes, compatibility with cell viability, and lack of solvent usage, allowing for printing of scaffolds in desired shapes and sizes that can support 3D cell culture(Dalton, 2017). In addition, incorporating the hydrogel as an extracellular matrix in these structures makes them more unique. Biomimetic, which is defined as Humanmade processes, substances, devices, or systems that imitate nature, covers a wide area from robots working in the body to systems that release drugs by making various measurements in the body. For the production of these devices, various metals must be present in their component. For this reason, it is important to examine the effects of metals in the body in such complex systems(Clegg et al., 2019; Mantha et al., 2019). In this study, metals such as Ti, Ta, Zr, Ag, W, and Nb, which are frequently used in biomaterial studies, were coated on the surface of the slide with 99.95% purity by the physical vapor deposition technique. This study, it was aimed to print scaffolds with the MEW technique on metal-coated surfaces and to determine the in vitro characterization properties of artificial soft tissues created with 3D bioprinting.