Determination of microbiological quality and histamine levels in rainbow trouts (Oncorhynchus mykiss)


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Aksoy A., Sezer C., ATASEVER M., Cetinkaya A., Gulbaz G.

JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.72, sa.4, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.12681/jhvms.29368
  • Dergi Adı: JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Anahtar Kelimeler: Rainbow trout, Microbial quality, Histamine, ELISA, PCR, YERSINIA-ENTEROCOLITICA STRAINS, BIOGENIC-AMINES, LISTERIA-MONOCYTOGENES, FISH, FOOD, IDENTIFICATION, SAFETY, FRESH
  • Atatürk Üniversitesi Adresli: Evet

Özet

This study aims to determine the microbiological quality and histamine levels of fresh-marketed rainbow trouts (Oncorhynchus mykiss). The total mesophilic aerobic bacteria (TMAB), total psychrophilic aerobic bacteria (TPAB), coliform, fecal coliform, Enterobacteriaceae, Pseudomonas, Staphylococcus-Micrococcus and mold-yeast numbers were detected as 4.24, 4.00, 1.10, 0.17, 1.50, 3.05, 0.28 and 3.82 log 10 cfu/g, respectively. Yersinia enterocolitica, Listeria monocytogenes, Escherichia coli O157:H7 and Vibrio spp bacteria were found in 18 (37.5%), 14 (29.1%), 8 (16.6%) and 14 (29.1%) respectively. Twenty-eight out of 51 Listeria isolates (54.9%) were verified through PCR analysis as L. monocytogenes. It was determined that the histamine level in the samples was above the detectable level (>2.5 mg/kg) in 8 of the samples (16.6%)determined using an ELISA method. It was concluded that the detection of foodborne pathogens in fresh-marketed fish samples potentially constitutes a public health hazard.