Effect of metformin/irinotecan-loaded poly-lactic-co-glycolic acid nanoparticles on glioblastoma: in vitro and in vivo studies

TAGHIZADEHGHALEHJOUGHI A., HACIMÜFTÜOĞLU A., ÇETİN M., UĞUR A. B., GALATEANU B., MEZHUEV Y., ...Daha Fazla

NANOMEDICINE, cilt.13, sa.13, ss.1595-1606, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 13
  • Basım Tarihi: 2018
  • Doi Numarası: 10.2217/nnm-2017-0386
  • Dergi Adı: NANOMEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1595-1606
  • Anahtar Kelimeler: brain tumor, Cavalieri's principle, irinotecan, metformin, nanoparticles, PLGA
  • Atatürk Üniversitesi Adresli: Evet

Özet

Aim: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. Methods: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). Results: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. Conclusion: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.