The association between insulin-like growth factors I, II and bovine papillomavirus type-1 expressions in naturally occuring bovine fibropapilloma cases


Alcigir M. E., TİMURKAN M. Ö.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.65, sa.2, ss.115-122, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1501/vetfak_0000002836
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.115-122
  • Anahtar Kelimeler: Bovine fibropapilloma, bovine papillomavirus type-1, insulin-like growth factors, CYTOLOGICAL ABNORMALITIES, BINDING-PROTEINS, CERVICAL SAMPLES, CANCER, RECEPTOR, PAPILLOMATOSIS, MITOGENESIS, SURVIVAL, VIRUS, CELLS
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, we hypothesized that insulin-like growth factors (IGFs) may have a combine effect together with BPV-1 in the etiopathogenesis of fibropapilloma. With this aim, macroscopic and histopathological findings of the fibropapillomatous changes were described in masses collected from the skin of neck, hindlimbs and abdominal region of 15 cattle, ranging from 14 to 23 months old. In several cases, hyperkeratosis (n=7) and/or parakeratosis (n=8), acanthosis (n=15), degenerative changes (n=15) and inflammatory cell infiltrations (n=9), and also vascularization (n=15) were accompanied to the neoplastic cells comprising paranchyme and stroma. In terms of virus entity in collected samples, L1 and L2 genes of BPV-1 were found to be positive by PCR. Immunohistochemically, BPV-1, IGF-I and IGF II expressions were found parallel. IGF-I positivities were stronger and more common in the epidermal cells and connective tissue components when compared with IGF-II positivity. In general, immunolocalization of both IGF-I and IGF-II were seen in the cytoplasm and membranes of all cell layers in the epidermis and only in the fibrocytes and fibroblasts in addition to collagen bundles. We concluded that IGF-I and IGF-II could have an effect as much as BPV-1 in pathogenesis of fibropapillomatous changes in the epidermis and dermis.