A SIMPLE UV AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF ANTIOXIDANT VITAMIN beta-CAROTENE IN SPIKED HUMAN PLASMA AND VITAMIN DRUG


DEMİRKAYA MİLOĞLU F., KADIOĞLU Y., ŞENOL O.

REVIEWS IN ANALYTICAL CHEMISTRY, cilt.29, ss.149-168, 2010 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29
  • Basım Tarihi: 2010
  • Dergi Adı: REVIEWS IN ANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.149-168
  • Atatürk Üniversitesi Adresli: Evet

Özet

A simple and sensitive UV, first- and second-order derivative spectrophotometric methods are proposed for the determination of beta-carotene in spiked human plasma and vitamin drug. The quantitative determination of beta-carotene was carried out by using wavelengths 452 nm (for UV; A), 496 nm (for first-order derivative; D-1) and 484 nm (for second-order derivative; D-2). The methods were validated with the concentration range 0.5-5.0 mu g/mL in both plasma and standard solution. The calibration curves were linear with correlation coefficients >0.999. The limits of quantitation of three methods were 0.075 mu g/mL in plasma and 0.050 mu g/mL in standard solution. For all methods, the recovery of beta-carotene from plasma and drug mixture was higher than 80.3% and 87.3%, respectively. The method was successfully applied to the determination of beta-carotene in pharmaceutical analysis and the results obtained from each of the three spectrophotometric methods in pharmaceutical analysis were statistically compared and no significant difference was found.