In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills

CEYHUN S. B., Senturk M., ERDOĞAN O., KÜFREVİOĞLU Ö. İ.

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, cilt.97, sa.3, ss.177-181, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 97 Sayı: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.pestbp.2010.01.003
  • Dergi Adı: PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.177-181
  • Anahtar Kelimeler: Carbonic anhydrase, Pesticide, Deltamethrin, Oncorhynchus mykiss, Gill, ERYTHROCYTE GLUTATHIONE-REDUCTASE, HISTOCHEMICAL-LOCALIZATION, KINETIC-PROPERTIES, METAL-IONS, PURIFICATION, INHIBITION, FRESH, WATER, FISH, LIVER
  • Atatürk Üniversitesi Adresli: Evet

Özet

Here we investigated the in vitro and in vivo effects of the pesticides. deltamethrin, diazinon, propoxur and cypermethrin, on the activity of rainbow trout (it) gill carbonic anhydrase (CA). The enzyme was purified from rainbow trout gills using Sepharose 4B-aniline-sulfanilamide affinity chromatography method. The overall purification was approx. 214-fold. SDS-polyacrylamide gel electrophoresis showed a single band corresponding to a molecular weight of approx. 29 kDa. The four pesticides dose-dependently inhibited in vitro CA activity. IC50 values for deltamethrin, diazinon, propoxur and cypermethrin were 0.137, 0.267, 0.420 and 0.460 mu M, respectively. In vitro results showed that pesticides inhibit rtCA activity with rank order of deltamethrin > diazinon > propoxur > cypermethrin. Besides, in vivo studies of deltamethrin were performed on CA activity of rainbow trout gill. rtCA was significantly inhibited at three concentrations (0.25, 1.0 and 2.5 mu g/L) at 24 and 48 h. (C) 2010 Elsevier Inc. All rights reserved.