Spectrofluorimetric Determination of alpha-Tocopherol in Capsules and Human Plasma

DEMIRKAYA-MILOGLU F., KADIOĞLU Y., ŞENOL O., YAMAN M.

INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.75, sa.5, ss.563-568, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 5
  • Basım Tarihi: 2013
  • Dergi Adı: INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.563-568
  • Anahtar Kelimeler: alpha-tocopherol, pharmaceutical, human plasma, cancer, spectrofluorimetric method, PERFORMANCE LIQUID-CHROMATOGRAPHY, REVERSED-PHASE HPLC, VITAMIN-E, CAROTENOIDS
  • Atatürk Üniversitesi Adresli: Evet

Özet

A simple, sensitive and rapid spectrofluorimetric method for determination of alpha-tocopherol in pharmaceutical capsule and human plasma was developed and validated. The native fluorescence of alpha-tocopherol was measured at 334 nm with excitation at 291 nm, after extraction of alpha-tocopherol from human plasma hexane: dichloromethane mixture. The calibration curves were linear (R >= 0.9993) in the concentration range of 0.25-2.5 mu g/ml of alpha-tocopherol in both standard solutions and plasma samples. The developed method was directly and easily applied for determination of alpha-tocopherol in the plasma of healthy volunteers and different type of bladder cancer and stomach cancer patients and also pharmaceutical capsule.