Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in Campylobacter jejuni in Calves
ANTIBIOTICS-BASEL, cilt.11, sa.4, 2022 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 11 Sayı: 4
- Basım Tarihi: 2022
- Doi Numarası: 10.3390/antibiotics11040531
- Dergi Adı: ANTIBIOTICS-BASEL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Veterinary Science Database, Directory of Open Access Journals
- Anahtar Kelimeler: Campylobacter, cattle, colonization, rectal feces, bovine respiratory disease (BRD), fluoroquinolone, danofloxacin treatment, antimicrobial resistance, pulsed-field gel electrophoresis, minimum inhibitory concentration, BOVINE RESPIRATORY-DISEASE, COMMERCIAL POULTRY FLOCKS, ANTIMICROBIAL RESISTANCE, THERMOPHILIC CAMPYLOBACTERS, FEEDLOT CATTLE, DAIRY-CATTLE, CIPROFLOXACIN RESISTANCE, MOLECULAR EPIDEMIOLOGY, ESCHERICHIA-COLI, STRAIN DIVERSITY
- Atatürk Üniversitesi Adresli: Evet
Özet
Campylobacter is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) Campylobacter in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in C. jejuni, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (n = 10), and were all inoculated orally with FQ-susceptible (FQ-S) C. jejuni; seven days later, Group 3 was challenged with transtracheal Mannheimia haemolytica, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R Campylobacter via culture. Before oral inoculation with C. jejuni, 87% of calves were naturally colonized by FQ-R C. jejuni. Two days after the inoculation, FQ-R C. jejuni decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all C. jejuni populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R C. jejuni populations following the injection was due mainly to enrichment of preexisting FQ-R C. jejuni, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant Campylobacter in cattle in response to FQ treatment.