Trolox Reduces Neuroblastoma-Induced Oxidative Stress and Inflammation
TURKISH NEUROSURGERY, vol.34, no.6, pp.1117-1121, 2024 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 34 Issue: 6
- Publication Date: 2024
- Doi Number: 10.5137/1019-5149.jtn.46442-24.4
- Journal Name: TURKISH NEUROSURGERY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, MEDLINE
- Page Numbers: pp.1117-1121
- Keywords: Inflammatory Neuroblastoma, Oxidative stress, Trolox
- Ataturk University Affiliated: Yes
Abstract
AIM: To determine the effects of different concentrations of Trolox on the cytotoxic, oxidant, antioxidant, and cytokine levels in the SH-SY5Y cell line. MATERIAL and METHODS: SH-SY5Y cells were grown in an appropriate medium and under appropriate conditions. Trolox was added to the cell line at concentrations of 50, 100, 200, 400 and 800 mu M and incubated for 24 and 48 hours. Subsequently, methyl thiazolyl tetrazolium (MTT) and proinflammatory cytokine level assays were performed, and the cytotoxicity of oxidative stress was assessed. RESULTS: Administration of 200 mu M of Trolox reduced the cancer cell viability to 77.76%. Furthermore, Trolox exhibited a concentration-dependent effect on the SH-SY5Y cell line. Administration of 200 mu M of Trolox also reduced the oxidant activity, increased the antioxidant capacity, and decreased the proinflammatory cytokine levels in neuroblastoma (NB) cells, which were consistent with the cytotoxicity test results. CONCLUSION: The results of this experimental study demonstrated a Trolox concentration of 200 mu M produces an anticancer effect on NB cell-line.