BOSWELLIA SERRATA PROTECTS AGAINST OLEIC ACID INDUCED-ARDS THROUGH THE INHIBITION OF OXIDATIVE STRESS AND ALTERATIONS OF BIOMOLECULAR COMPOSITIONS

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Şimşek Özek N., Aysin F., Koç K.

3. ULUSLARARASI MARMARA BİLİMSEL ARAŞTIRMALAR VE İNOVASYON KONGRESİ, İstanbul, Türkiye, 19 - 20 Kasım 2022, ss.674-683

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.674-683
  • Atatürk Üniversitesi Adresli: Evet

Özet

Acute respiratory distress syndrome (ARDS) is a serious lung injury characterized by the rapid onset of

widespread inflammation. There has been a strong conflict between folk and modern medicine for a long time.

Boswellia serrata (B. serrata) is one of the prime examples of medicinal plants with antioxidant, antiinflammatory,

anti-cancer, immunomodulation, and other biological properties. The objective of this study is to

investigate the protective role of the extract of B. serrata on Oleic acid-induced ARDS in rats. The animal model

was established by intravenous injecting rats with a single dose of Oleic acid. Animals were randomly divided

into three groups: I. control, II. ARDS, III. ARDS + B. serrata. B. serrata extract (1000 mg/kg) was

administered orally for two weeks in Group II rats. After the last gavage, a single dose of Oleic acid was given by

intravenous injecting to Group II and waited for 24 hours. Then, rats were sacrificed with sevoflurane, and their

lung was removed. We determined the levels of Interferon-gamma (IFN-γ), Total antioxidant capacity (TAC),

total oxidant status (TOS), superoxide dismutase (SOD), and Catalase (CAT) by ELISA in lung tissue. The

biomolecular compositional changes were determined from FTIR spectroscopy together with chemometric

analysis.

The levels of IFN-γ and TOS were significantly lower in the ARDS + B. serrata group compared to the ARDS

group. Moreover, significant increases in the levels of TAS, SOD, and CAT were found in B. serrata

administered group with respect to the ARDS group. Spectral analysis revealed significant alterations in the

lipids and nucleic acid amounts in the ARDS group and B. serrata pre-treatment prevented the formation of these

changes. These findings suggest that the B. serrata has a lung protective effect through the inhibition of oxidative

stress, and regulation of IFN-γand biomolecule levels in the ARDS model.