Genomic characterization, antimicrobial resistance profiles, enterotoxin, and biofilm production of methicillin-resistant Staphylococcus aureus isolated from clinical and animal products origins in Eastern Turkey

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BARAN A., ÖZ C., CENGİZ S., ADIGÜZEL M. C.

PESQUISA VETERINARIA BRASILEIRA, vol.42, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 42
  • Publication Date: 2022
  • Doi Number: 10.1590/1678-5150-pvb-6991
  • Journal Name: PESQUISA VETERINARIA BRASILEIRA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: Genome, antimicrobial resistance, enterotoxin, biofilm, methicillin resistance, Staphylococcus aureus, animal products, Turkey, enterobacterial repetitive intergenic consensus, ERIC-PCR, multi locus sequence typing, MLST, ANTIBIOTIC-RESISTANCE, DAIRY-PRODUCTS, MOLECULAR CHARACTERIZATION, RAW-MILK, PREVALENCE, BOVINE, GENES, FARMS, IDENTIFICATION, DIVERSITY
  • Ataturk University Affiliated: Yes

Abstract

Staphylococcus aureus is an opportunistic and ubiquitous pathogen found in the skin, nares, and mucosal membranes of mammals. Increasing resistance to antimicrobials including methicillin has become an important public concern. One hundred and eight (108)S. aureus strains isolated from a total of 572 clinical and animal products samples, were investigated for their biofilm capability, methicillin resistance, enterotoxin genes, and genetic diversity. Although only one strain isolated from raw retail was found as a strong biofilm producer, the percentage of antimicrobial resistance pattern was relatively higher. 17.59% of S. aureus strains tested in this study were resistant to cefoxitin and identified as methicillin-resistant S. aureus (MRSA) isolates. mecA and mecC harboring S. aureus strains were detected at a rate of 2.79% and 0.93%, respectively. In addition, staphylococcal enterotoxin genes including Sea, Seb, Sec, and Sed genes were found to be 18.5%, 32.4%, 6.5% and 3.7%, respectively. The phylogenetic relationship among the isolates showed relationship between joint calf and cow milk isolates. Multi locus sequence typing (MLST) revealed three different sequence types (STs) including ST84, ST829, and ST6238. These findings highlight the development and spread of MRSA strains with zoonotic potential in animals and the food chain throughout the world