A pilot study on feline astrovirus and feline panleukopenia virus in shelter cats in Erzurum, Turkey


AYDIN H., TİMURKAN M. Ö.

REVUE DE MEDECINE VETERINAIRE, cilt.169, ss.52-57, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 169
  • Basım Tarihi: 2018
  • Dergi Adı: REVUE DE MEDECINE VETERINAIRE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.52-57
  • Anahtar Kelimeler: Diarrhoea, feline astrovirus, feline panleukopenia virus, molecular characterization, Turkey, CANINE-PARVOVIRUS, GENETIC-ANALYSIS, INFECTION, EMERGENCE, EVOLUTION
  • Atatürk Üniversitesi Adresli: Evet

Özet

The aim of the study was to investigate the presence and molecular character of feline astrovirus and feline panleukopenia virus in cats in Erzurum, Turkey. Fifty feces from cats of which 13 with clinical signs and 37 with clinically healthy were analysed using reverse transcription polymerase chain reaction for ORF2 gene of astrovirus and VP2 gene of panleukopenia virus. Upon reverse transcription polymerase chain reaction, sequence analyses, bioinformatics analyses and phylogeny was performed. Of the cats 8 % (4/50) were positive for FAstV and 10 % (5/50) were positive for FPLV. Stool samples from the astrovirus and panleukopenia virus positive cats were negative for rotavirus and coronavirus. When positive astrovirus amplicons were sequenced, the feline astrovirus strains appeared to be genetically more related to human astroviruses than to porcine, canine, bat, and avian astroviruses. Result of the sequence analysis of the feline panleukopenia virus positive PCR products indicate that it was closely related to Asian strains in the cluster of G1. This study also has shown that the genotypes of the feline panleukopenia virus found in this study were different from previously reported a single study from Turkey and were closely related to European strains.