Acrylamide (ACR) is a heat-induced carcinogen substance that is found in some foods due to cooking or other
thermal processes. The aim of present study was to assess the probable protective effects of morin against ACRinduced
hepatorenal toxicity in rats. The rats were treated with ACR (38.27 mg/kg b.w., p.o.) alone or with
morin (50 and 100 mg/kg b.w., p.o.) for 10 consecutive days. Morin treatment attenuated the ACR-induced liver
and kidney tissue injury by diminishing the serum AST, ALP, ALT, urea and creatinine levels. Morin increased
activities of SOD, CAT and GPx and levels of GSH, and suppressed lipid peroxidation in ACR induced tissues.
Histopathological changes and immunohistochemical expressions of p53, EGFR, nephrin and AQP2 in the ACRinduced
liver and kidney tissues were decreased after administration of morin. In addition, morin reversed the
changes in levels of apoptotic, autophagic and inflammatory parameters such as caspase-3, bax, bcl-2, cytochrome
c, beclin-1, LC3A, LC3B, p38α MAPK, NF-κB, IL-1β, IL-6, TNF-α and COX-2 in the ACR-induced toxicity.
Morin also affected the protein levels by regulating the PI3K/Akt/mTOR signaling pathway and thus alleviated
ACR-induced apoptosis and autophagy. Overall, these findings may shed some lights on new approaches for the
treatment of ACR-induced hepatotoxicity and nephrotoxicity.