Evaluation of 8-hydroxy-2-deoxyguanosine and NFkB activation, oxidative stress response, acetylcholinesterase activity, and histopathological changes in rainbow trout brain exposed to linuron


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TOPAL A., ALAK G., ALTUN S., Erol H. S., ATAMANALP M.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, cilt.49, ss.14-20, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.etap.2016.11.009
  • Dergi Adı: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.14-20
  • Anahtar Kelimeler: Pesticide, Linuron, Fish, 8-hydroxy-2-deoxyguanosine, NFkB, Toxicity, Oxidative stress, Histopathology, AChE, LONG-TERM EXPOSURE, KAPPA-B, TRANSCRIPTION FACTOR, ANTIOXIDANT ENZYMES, LIPID-PEROXIDATION, CHANNA-PUNCTATUS, DNA-DAMAGE, IN-VITRO, LIVER, HERBICIDE
  • Atatürk Üniversitesi Adresli: Evet

Özet

Linuron is a widely used herbicide to control grasses and annual broad leaf weeds. It is known that linuron has toxic effects on different organisms. However, the toxic effects of linuron on aquatic organisms, especially fish, is completely unknown. Thus, we aimed to investigate changes in 8-hydroxy2-deoxyguanosine (8-OHdG) and nuclear factor kappa B (NFkB) activity, histopathological changes, antioxidant responses and acetylcholinesterase (AChE) activity in rainbow trout brain after exposure to linuron. Fish were exposed to 30 mu g/L, 120 mu g/l. and 240 mu g/L concentrations of linuron for twenty-one days. Brain tissues were taken from fish for 8-OHdG and NFkB activity, histopathological examination and determination of superoxide dismutase (SOD), catalase (CAT) enzyme activity, lipid peroxidation (LPO), and reduced glutathione (GSH) levels. Our data indicated that high linuron concentrations caused a decrease in GSH levels, SOD and CAT activities in brain tissues (p <0.05). LPO levels were significantly increased by 240 mu g/L linuron. All concentrations caused a significant inhibition in brain AChE enzyme activity (p < 0.05). Immunopositivity was detected for 8-OHdG and NFkB, and linuron exposure caused histopathological damage to the brain tissues. The results of this study can provide useful information for understanding of linuron-induced toxicity. (C) 2016 Elsevier B.V. All rights reserved.