Effects of Helichrysum plicatum extract against Thallium I sulphate induced reproductive damage in male rats


Ömür A. D. , Apaydın Yıldırım B. , Küçükler S.

Faseb Journal, vol.30, 2016 (Journal Indexed in SCI)

  • Publication Type: Article / Abstract
  • Volume: 30
  • Publication Date: 2016
  • Title of Journal : Faseb Journal

Abstract

In this study, 24 male Sprague Dawley rats aged eight weeks old and weighted 250–300 gr, were used in this experiment. The animals were obtained from Atatürk University Experimental Research Centre and housed in standard laboratory conditions. Commercial pellet chow and fresh drinking water were available ad libitum. Rats were divided into four groups. I. group (n= 6) referred as control group and physiological saline intraperitonally (IP) to the animals. II. group (n=6) referred as 10-mg/kg Thallium Sulphate (TS) was injected IP to animals. III. Group (n=6) was referred as TS + HPE, 10-mg/kg TS injected IP and 100 mg/kg HPE peros to animals. IV. group (n=6) was referred as HPE, 100 mg/kg HPE peros to animals. The treatment was performed for consecutive 8 days. The animals were tranquilized and sacrificed on 9ht day of study. Cauda epididymal semen samples and testes tissues were collected. Routine semen examinations were performed and oxidative stress levels of testicular tissues were assayed. Motility in TS group was significantly lower when compared to control and experimental groups. Head and tail abnormality in TS and HPE+TS group were statistically higher when compared to control and HPE groups. MDA level of TS group was significantly higher than in control and treatment groups. Glutathione peroxidase (GPx) and glutathione (GSH) levels in TS group were lower than the other groups. The lowest SOD level was in TS group while the highest level was in control group. MDA level was higher and CAT level was lower in TS group when compared to other groups.

Helichrysum plicatum extract is effective on mentioned spermatological and plasma /testis tissue biochemical parameters against Thallium(I) sulphate in terms of testicular toxicity. There are statistically significant differences between mentioned groups as can be seen in the above.