Herbal products have been used in textile dyeing since ancient times. Based on current consumer demands, use of natural dyes in dyeing is increasing once again. The main goal of this study is to investigate the metameric properties of natural dyes, an important issue for textile dyeing. For this aim, wool fabrics were dyed with different natural dyes according to the pre-mordanting method. Spectral measurements of dyed samples were performed and color changes under different illuminants were assessed for metamerism. It was statistically observed that the type of natural dye, dye concentration, and the type of mordanting agent were responsible for metamerism to different extents. From the results, madder showed the greatest metamerism, while alkanet root showed the least.