Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on alpha-glycosidase, alpha-amylase and human glutathione S-transferase enzymes

GÜLÇİN İ., TASLIMI P., Aygun A., SADEGHIAN N., BASTEM E., Kufrevioglu Ö. İ., ...Daha Fazla

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.119, ss.741-746, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 119
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.ijbiomac.2018.08.001
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.741-746
  • Anahtar Kelimeler: Antidiabetic, Antiparasitic, Glutathione S-transferase, Enzyme inhibition, LIGHT PHOTOCATALYTIC DEGRADATION, METABOLITES VULPINIC ACID, IN-VITRO ANTIOXIDANT, CARBONIC-ANHYDRASE, GLUCOSIDASE INHIBITORS, ARTEMISIA PLANTS, USNIC ACID, ACETYLCHOLINESTERASE, EXPRESSION, PI
  • Atatürk Üniversitesi Adresli: Evet

Özet

The glutathione S-transferase (GST) was purified from fresh blood erythrocytes using affinity column chromatography. Also, alpha-amylase from porcine pancreas and alpha-glycosidase from Saccharomyces cerevisiae were used as target enzymes. In this study, these compounds were tested on alpha-amylase, alpha-glycosidase, and GST enzymes and demonstrated effective inhibitor compounds with K-i values in the range of 8.34-40.78 mu M against GST, and 120.53-892.36 nM against alpha-glycosidase. Additionally, the phenolic molecules were tested for the inhibition of alpha-amylase enzyme which determined effective inhibition profile with IC50 values in the range of 175.01-626.58 nM. Indeed, these molecules can be elective inhibitors of GST, alpha-glycosidase and alpha-amylase enzymes as antidiabetic and antiparasitic agents. (C) 2018 Elsevier B.V. All rights reserved.