Akademik Veri Yönetim Sistemi
İSTANBUL INTERNATIONAL MODERN SCIENTIFIC RESEARCH CONGRESS, İstanbul, Türkiye, 4 - 05 Haziran 2021, ss.326-334
Lactic acid bacteria (LAB), which form the general flora of the digestive system, are
frequently found in food products such as meat, vegetables, milk and yogurt. The most
powerful way to obtain strains useful for the food industry and to produce medicinally
important products is the isolation and screening of LAB from these foods. In our present
study; 50 isolates were obtained from 15 raw milk samples brought from various regions of
Turkey and these isolates were phenotypically characterized at the first stage. Isolates, whose
morphological and physiological analyzes were made, were then distinguished from each
other on the basis of species by genomic fingerprint analysis. The antimicrobial properties of
13 isolates, which were thought to be different as a result of the analysis, were tested by disk
diffusion method. The isolate showing strong antimicrobial effect was first analyzed
biochemically with API 50 CHL System (bioMérieux, France) and then genomically
identified by 16S rRNA sequence analysis. As a result of the study, it was determined that the AT12 coded isolate had a strong antimicrobial effect. As a result of the 16S rRNA sequence
analysis, it was detected that the AT12 coded isolate was 99% similar to Levilactobacillus
brevis.