2nd Universal Conference on Multidisciplinary Research & Innovation. UPACRI 2021 – July 24 & 25, 2021. Organized by DHS FOUNDATION, New Delhi, INDIA., New Delhi, India, 24 - 25 July 2021, pp.1-2
The aim of this study was to determine the efficacy of photo-stimulation against bovine sperm cells was evaluated by red laser diode (640 nm) in 5 mw. Spermatological parameters were evaluated in terms of motility and membrane integrity. All sperm samples were obtained from bulls by artificial vagina. Semen was diluted to a final concentration of 108 spermatozoa per ml in 0.50 ml straws. Sperm cells were frozen using the conventional method. The straws were kept in the water bath for 30 seconds at 37 ° C. Regarding the application of red laser diode -640 nm- in 5 mw, there was no statistically differences between the samples before irradiation and after irradiation at the 2-1-3 regime ( 2; 2 minutes irradiation, 1; 1 minute dark condition, 3; 3 minutes irradiation) in terms of motility (p-value ˃ 0.05). On the other hand, when we evaluate the membran integrity, there was statistically differences between the samples before irradiation and after irradiation at the same regime (p-value ≤ 0.05).
As a result it was determined that the application of red laser diode -635 nm- in 5 mw at the regime of 2-1-3, protects the membrane integrity of frozen-thawed bull sperm when applied upon thawing.
Key Words: Bull sperm cell, red laser diode, photostimulation