Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) Determination of Allergenic Proteins in Edible Flour Derived from Yellow Mealworms

Papastavropoulou K., Mavrakaki S., Kostakis M., Koupa A., Thomaidis N. S., Calokerinos A. C., ...Daha Fazla

Analytical Letters, cilt.58, sa.18, ss.3176-3192, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 18
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/00032719.2025.2456683
  • Dergi Adı: Analytical Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3176-3192
  • Anahtar Kelimeler: High–performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), insect allergenic proteins, tropomyosin, yellow mealworm allergens
  • Atatürk Üniversitesi Adresli: Evet

Özet

Entomophagy offers significant benefits but also poses allergen risks. This study examined the presence of allergenic proteins in freeze-dried yellow mealworm powder using LC-MS/MS with electrospray ionization in positive mode. Allergenic peptides were identified and compared with plant- and animal-based allergens listed under EU Regulation 1169/2011, utilizing certified reference materials. The findings reveal the presence of tropomyosin, which may trigger allergic reactions in individuals with crustacean allergies. The larvae’s diet did not significantly influence allergen content. This research underscores the need for robust legislation to ensure safety in insect production and consumption, supporting the growth of the edible insect industry.