POSITIVE EFFECTS OF OXYRESVERATROL ON OXIDATIVE STRESS DAMAGE IN L929 FIBROBLAST CELLS OKSİRESVERATROLÜN L929 FİBROBLAST HÜCRELERİNDEKİ OKSİDATİF STRES HASARI ÜZERİNE OLUMLU ETKİLERİ


Creative Commons License

Ateş F. M., BAYIR Y.

Ankara Universitesi Eczacilik Fakultesi Dergisi, vol.47, no.2, pp.1-14, 2023 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.33483/jfpau.1246570
  • Journal Name: Ankara Universitesi Eczacilik Fakultesi Dergisi
  • Journal Indexes: Scopus, Central & Eastern European Academic Source (CEEAS), EMBASE, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1-14
  • Keywords: OSI, oxyresveratrol, TAC, TOC, TPS, xCELLigence
  • Ataturk University Affiliated: Yes

Abstract

Objective: It was aimed to determine the regenerative effect of oxyresveratrol in eliminating the cell damage caused by the effect of H2O2 in L929 fibroblast cells. Material and Method: Total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), total phenolic substance levels of oxyresveratrol were measured at different concentrations. After the IC50 value of oxyresveratrol fibroblast cells was determined by the MTT method, the regenerative effect on oxidative stress damage induced by H2O2 at 12.5-400 µM concentrations was performed with the xCELLigence device to measure cell proliferation in-vitro. In addition, scratch analysis was performed at concentrations of 3.125-25 µM in order to determine the wound healing levels in cell damage. Result and Discussion: The TAC value of oxyresveratrol at 0.5 mg/ml was 3 ± 0.3 and the TOC value was 0.77 ± 0.52; (OSI) value was found to be 0.02 ± 0.09. It has been observed that the total phenolic substance (TPS) concentrations of oxiresveratrol at different doses from 0.625 mg/ml to 10 mg/ml have higher TPS from low concentration to high concentration. Oxyresveratrol L929 fibroblast cells IC50 value 214.2 μM; The IC50 value of oxyresveratrol was determined to be 109.7 μM in the cell line of L929 fibroblast cells exposed to H2O2. The highest cell proliferation was observed when measuring the 12.5 μM concentration of oxiresveratrol with the xCELLigence device. Scratch analysis showed a greater improvement than other doses, with a cell proliferation of 62% at the 24th hour and 88% at the 48th hour at a concentration of 12.5 μM oxyresveratrol in H2O2-damaged cells. The 12.5 μM concentration was determined to be the most effective concentration in both proliferation and scratch analysis. Oxyresveratrol; with its antioxidant capacity at low concentrations, L929 protects fibroblast cells from oxidative stress in cellular damage caused by H2O2, and creates a power multiplier effect on fibroblast viability and migration. These results indicate that oxyresveratrol; showed that it can be effective at the cellular level preventing of acute or chronic diseases caused by free oxygen radicals. It can be thought that oxyresveratrol may be a potential molecule in the treatment of oxidative stress-induced diseases with more comprehensive studies to be carried out at the cellular level and in-vivo studies.