Relationships Among some Pears Genotypes (Pyrus Communis L.) Based on ISSR and RAPD Analysis

Kalkisim O., Okcu M., Okcu Z., Karabulut B., Yildirim N., Ağar G.

ERWERBS-OBSTBAU, cilt.58, sa.4, ss.259-264, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s10341-016-0287-5
  • Dergi Adı: ERWERBS-OBSTBAU
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.259-264
  • Anahtar Kelimeler: Pyrus, ISSR, RAPD, Genetic diversity, Pear, MOLECULAR CHARACTERIZATION, MARKERS
  • Atatürk Üniversitesi Adresli: Evet

Özet

Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 RAPD and ISSR primer investigated, 22 could amplify clearly and consistently. Cluster analysis of the pears genotypes was performed based on data from polymorphic bands RAPD and ISSR by using Jaccard's similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. The 31 pear genotypes were classified into two major groups. Cluster A was divided into 2 subclusters: Gumushane pears and Trabzon pears. Cluster B consisted of Artvin pears. The similarity matrix values ranged between 0.105 and 0.968.