Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis

Choi S. W., Oh H., Park S. Y., Cho W., HASSIBELNABY A. M. A., HACIMÜFTÜOĞLU A., ...More

Biochemical and Biophysical Research Communications, vol.642, pp.113-117, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 642
  • Publication Date: 2023
  • Doi Number: 10.1016/j.bbrc.2022.12.056
  • Journal Name: Biochemical and Biophysical Research Communications
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.113-117
  • Keywords: Musclin, Myokine, Adipocyte, Obesity, Lipogenesis, Lipolysis, SKELETAL-MUSCLE, PROTEIN, DIFFERENTIATION, RESISTANCE, OSTEOCRIN, EXERTS, FAT
  • Ataturk University Affiliated: Yes

Abstract

© 2022 Elsevier Inc.Musclin (MUS), an exercise-responsive myokine, has been documented to attenuate inflammation and enhance physical endurance. However, the effects of MUS on differentiation and related molecular mechanisms in adipocytes have not yet been studied. In this study, we found that treatment with MUS attenuated lipid accumulation in fully differentiated 3T3-L1 cells. Furthermore, MUS treatment enhanced lipolysis assessed by glycerol release, and caused apoptosis, whereas it reduced the expression of lipogenic proteins, such as PPARγ and processed SREBP1. Treatment with MUS augmented phosphorylated PKA expression, whereas suppressed p38 phosphorylation in 3T3-L1 adipocytes. H89, a selective PKA inhibitor reduced the effects of MUS on lipogenic lipid accumulation as well as lipolysis except for apoptosis. These results suggest that MUS promotes lipolysis and suppresses lipogenesis through a PKA/p38-dependent pathway, thereby ameliorating lipid deposition in cultured adipocytes. The current study offers the potential of MUS as a therapeutic approach for treating obesity with few side effects.