Quantification of artesunate and its metabolite, dihydroartemisinin, in animal products using liquid chromatography–tandem mass spectrometry

Zheng W., Abd El-Aty A. M., Kim S., Choi J., HACIMÜFTÜOĞLU A., Shim J., ...More

Journal of Separation Science, vol.41, no.18, pp.3538-3546, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 18
  • Publication Date: 2018
  • Doi Number: 10.1002/jssc.201800499
  • Journal Name: Journal of Separation Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3538-3546
  • Keywords: aquaculture, artesunate, dihydroartemisinin, porcine muscle, sample preparation, SAMPLE PREPARATION APPROACH, SOLID-PHASE EXTRACTION, MULTI-RESIDUE METHOD, HUMAN PLASMA, PESTICIDE-RESIDUES, FOOD-PRODUCTS, VALIDATION, COMBINATION, VEGETABLES, TOXICITY
  • Ataturk University Affiliated: Yes

Abstract

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimAn analytical approach using a modified quick, easy, cheap, effective, rugged, and safe extraction method followed by liquid chromatography with electrospray ionization tandem mass spectrometry was developed herein for the determination of artesunate and its metabolite, dihydroarteminsinin in porcine muscle, egg, eel, flatfish, and shrimp. 10% trichloroacetic acid in acetonitrile mixed with ethyl acetate was used as an extraction solvent. To obtain a good separation, a Phenomenex Kinetex reversed-phase analytical column was selected with mobile phase consisting of distilled water (A) and acetonitrile (B), both containing 0.05% formic acid. Good linearity was achieved using matrix-matched calibrations constructed from six concentrations (5–50 μg/kg) with determinant coefficients ≥0.9918. Recoveries estimated from three spiking concentrations (5, 10, and 20 μg/kg) ranged between 71.3 and 104.7% in all matrixes with relative standard deviations ≤8.3%. A variety of samples purchased from markets in Seoul were tested following the protocol described herein. The artesunate and dihydroarteminsinin were not detected in any matrix. The methodology proposed could be used for routine determination of artesunate and its metabolite, dihydroartemisinin in various animal products having variable percentages of fat and protein.