Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Park S., Park C., Lee S. J., Cha B., Cho Y. A., Song Y., ...More

Biomedical Chromatography, vol.29, no.4, pp.612-618, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 4
  • Publication Date: 2015
  • Doi Number: 10.1002/bmc.3322
  • Journal Name: Biomedical Chromatography
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.612-618
  • Keywords: Bupropion, Escitalopram, LC/MS/MS, Major depressive disorder, Quetiapine
  • Ataturk University Affiliated: Yes

Abstract

© 2014 John Wiley & Sons, Ltd. In the present study, an effective high performance liquid chromatography-tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C 18 column using reversed-phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3→184.2 for BUP, 384.2→253.1 for QUE, 325.3→109.3 for ESC and 227.2→181.2 for the internal standard. The method showed good linearity (R 2 ≥0.997), precision (relative standard deviation ≤7.5%), satisfactory intra- and interday accuracy (88.4-113.0%) and acceptable extraction recovery (87.2-115.0%), matrix effect (84.5.5-108.7%) and stability (92.3-103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples.