In vitro effects of calcium gluconate, potassium chloride and sodium bicarbonate on human erythrocyte glucose 6-phosphate dehydrogenase Kalsiyum glukonat, potasyum klorür ve sodyum bikarbonat'in i̇nsan eritrosit glukoz 6-fosfat dehidrogenaz enzimi üzerine in vitro etkileri

Altikat S., Büyükokuroǧlu M. E., ÇİFTÇİ M., BAKAN N., KÜFREVİOĞLU Ö. İ.

Ondokuz Mayis Universitesi Tip Dergisi, cilt.19, sa.1, ss.39-44, 2002 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 1
  • Basım Tarihi: 2002
  • Dergi Adı: Ondokuz Mayis Universitesi Tip Dergisi
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.39-44
  • Atatürk Üniversitesi Adresli: Evet

Özet

Effects of some electrolytes which are frequently used for treatment, on erythrocyte glucose 6-phosphate dehydrogenase enzyme (G6PD) activity were investigated in in vitro conditions. G6PD was purified by affinity chromatography. Enzyme activity was determined according to the Beutler method by using a spectrophotometer at 340 nm. According to the results of study, 50% inhibitor drug concentration values (I50) were 19.4 mM for calcium gluconate and 160 mM for sodium bicarbonate in in vitro study. There was not observed any inhibitor effect of potassium chloride on glucose 6-phosphate dehydrogenase activity. The results of this study revealed that calcium gluconate and sodium bicarbonate have significant inhibition effect but no potassium chloride on the activity of G6PD enzyme in in vitro.