New xylanolytic enzyme from Geobacillus galactosidasius BS61 from a geothermal resource in Turkey


Sari B., FAİZ Ö., Genc B., ŞİŞECİOĞLU M., ADIGÜZEL A., Adiguzel G.

International Journal of Biological Macromolecules, cilt.119, ss.1017-1026, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 119
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.ijbiomac.2018.07.166
  • Dergi Adı: International Journal of Biological Macromolecules
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1017-1026
  • Anahtar Kelimeler: Thermophilic, 16S rRNA sequencing, BOX-PCR, Cloning, Geobacillus galactosidasius BS61, Xylanase, THERMOSTABLE ENDO-XYLANASE, THERMOPHILIC BACTERIA, SP-NOV., GENERATING XYLOOLIGOSACCHARIDES, BACILLUS-SUBTILIS, CELLULASE-FREE, FRUIT JUICE, PURIFICATION, STRAIN, CLONING
  • Atatürk Üniversitesi Adresli: Evet

Özet

In this study, isolation, conventional and molecular characterizations of ten thermophilic bacteria from Rize/Ayder were carried out. Xylanase from Geobacillus galactosidasius BS61 (GenBank number: 10(447660) was purified by acetone precipitation, Diethylaminoethyl-cellulose and Sephadex G-100 chromatographies. The xylanase of G. galactosidasius BS61 in clarifying fruit juice was also investigated. Enzyme was purified 29.80-fold with 75.18% yield; and molecular weight was determined as 78.15 kDa. The optimum temperature of xylanase was 60 degrees C. The enzyme activity was maintained fully after 24 h and over 50% after 168 h at pH 4.0-10.0, while optimum pH was 7.0. K-m and V-max for beech wood xylan were measured as 3.18 mg mL(-1), 123 U mg protein(-1). In addition, Ca2+, Na+, Al3+, Zn2+, Cd2+, Mg2+, Ni2+, Cu2+ had decreasing effect on enzyme activity, while enzyme activity had been protected against anions, especially HSO3- and HPO42- stimulated enzyme activity. Xylanase applications (with 15 U/mL enzyme activity) in orange and pomegranate juices were increased; and the sugar and turbidity amounts were reduced 17.36% +/- 1.18 and 30.52 +/- 1.23, respectively. These results indicated that the xylanase of G. galactosidasius BS61 has biotechnological potential in juice clarification due to its stability against metal ions, chemicals and high pH-values. (C) 2018 Elsevier B.V. All rights reserved.