Methionine supplementation improves ram sperm parameters during liquid storage at 5 degrees C

Bucak M. N., Çoyan K., Öztürk C., Güngör Ş., Ömür A. D.

CRYOBIOLOGY, cilt.65, sa.3, ss.335-337, 2012 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.cryobiol.2012.09.002
  • Dergi Adı: CRYOBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.335-337
  • Anahtar Kelimeler: Ram sperm, Methionine, Lipoic acid, Liquid storage, Fluorescent staining, ALPHA-LIPOIC ACID, MITOCHONDRIAL-FUNCTION, LIPID-PEROXIDATION, SEMEN, MOTILITY, SPERMATOZOA, VIABILITY
  • Atatürk Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the effects of methionine and lipoic acid on ram sperm parameters during liquid storage (5 degrees C). Ejaculates collected from five Merino rams were pooled at 37 degrees C. Each pooled ejaculate was divided into five equal aliquots and diluted (37 degrees C) with five extenders, one of which was without additives, two of which contained methionine at two different doses, and the other two of which contained lipoic acid at two different doses. Sperm parameters were determined at 0, 24, 48, 72 and 96 h of liquid storage at 5 degrees C.