Tnf-alpha inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy


Saritemur M., Un H., ÇADIRCI E. , Karakus E. , AKPINAR E. , HALICI Z. , ...More

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, vol.39, no.2, pp.577-588, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1016/j.etap.2015.01.002
  • Title of Journal : ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Page Numbers: pp.577-588
  • Keywords: Contrast-media, Nephrotoxicity, Infliximab, Rat, ACUTE-RENAL-FAILURE, ACUTE KIDNEY INJURY, CISPLATIN NEPHROTOXICITY, CELL-DEATH, RATS, MEDIA, EXPRESSION, MODEL, PRETREATMENT, INFLAMMATION

Abstract

Contrast medium-induced nephropathy (CIN) remains as a problem with high incidence and mortality rates. The aim of this study is to examine the roles of infliximab (INF) in the glycerol (GLY) and CIN model in rats. The rats were separated into five groups (n=8): Healthy, GLY, GLY+CM, GLY+CM+INF 5 mg/kg intraperitoneally (i.p.), and GLY+CM+INF 7 mg/kg (i.p.). Antioxidant levels in the therapy groups were observed to be quite similar to those in the healthy group. In this study, while the kidney TNF-alpha, TGF-1 beta and Caspase 3 gene expressions' levels increased in the nephrotoxic groups, these levels were found to have decreased in the treatment groups. Moreover, histopathologic examination showed that hyaline, haemorrhagic casts and necrosis were increased in nephrotoxicity group, whereas they decreased in the therapy group. Furthermore, TNF-alpha and NF-kappa B expression were decreased with infliximab administrated groups similar to control group. In conclusion, we suggest that infliximab have protective roles on CIN. (C) 2015 Elsevier B.V. All rights reserved.