The effect of mineral nutrient application on freezing tolerance in red cabbage, carrot, and spinach under cold-acclimated and unacclimated conditions were evaluated in this study. Effects of nutrient-solution application and cold acclimation on temperature at which 50% of leaves are injured (LT50), values, and ice nucleation activity in all of the plants were found to be statistically significant. Nutrient-solution applications decreased LT50 values in all of the species under unacclimated and cold-acclimated conditions. Freezing injuries in cold-acclimated plants were significantly lower than in unacclimated plants in all of the nutrient application doses. In addition to this, 600-900 mg l(-1) nutrient solutions also gave low plant injury in all conditions. Nutrient-solution applications increased ice nucleation temperatures of apoplastic proteins, proline, and total chlorophyll contents in all of the plant species under unacclimated and cold-acclimated conditions. Among the plant species, carrot was the most resistant plant to freezing injury under all nutrient application doses in the tested conditions.