Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway

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Koç K., Şimşek Özek N., Aysin F., Demir Ö., Yılmaz A., Yılmaz M., ...More

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, vol.34, no.2, pp.755-766, 2024 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2024
  • Doi Number: 10.1080/09603123.2023.2166023
  • Journal Name: INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, CINAHL, Educational research abstracts (ERA), EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.755-766
  • Keywords: Hispidulin, lung injury with ARDS, ACE activity, RESPIRATORY-DISTRESS-SYNDROME, ACUTE LUNG INJURY, OXIDATIVE STRESS, FATTY-ACIDS, PATHOGENESIS, MICE, APOPTOSIS
  • Open Archive Collection: AVESIS Open Access Collection
  • Ataturk University Affiliated: Yes

Abstract

This study investigates the protective role of Hispidulin on acute respiratory distress syndrome (ARDS) in rats. Rats were divided into three groups: control, ARDS, ARDS+ Hispidulin. The ARDS models were established by injecting rats with oleic acid. Hispidulin (100 mg/kg) was injected i.p. an hour before ARDS. Myeloperoxidase (MPO), Interleukin-8 (IL-8), Mitogen-activated protein kinases (MAPK), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD), Glutathione (GSH), and Angiotensin-converting enzyme (ACE) were determined by ELISA. Tumor necrosis factor-alpha (TNF-alpha) expression was described by RT-qPCR. Caspase-3 immunostaining was performed to evaluate apoptosis. Compared with the model group, a significant decrease was observed in the MPO, IL-8, MAPK, ACE, LPO levels, and TNF-alpha expression in the ARDS+ Hispidulin group. Moreover, reduced caspase-3 immunoreactivity and activity of ACE were detected in the Hispidulin+ARDS group. The protective effect of Hispidulin treatment may act through inhibition of the ACE activity and then regulation of inflammatory cytokine level and alteration of apoptosis.