Development of a single-step precipitation cleanup method for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma

Choi J., Mamun M. I. R., HASSIBELNABY A. M. A., Park J., Shin E., Park J. Y., ...Daha Fazla

FOOD CHEMISTRY, cilt.127, sa.4, ss.1878-1883, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 127 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.foodchem.2011.02.027
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1878-1883
  • Atatürk Üniversitesi Adresli: Hayır

Özet

In this study, we describe a newly developed and simple analytical method using high-performance liquid chromatography coupled to fluorescence detector (HPLC-FLD) for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma. A single-step sample preparation, including extraction with acidic acetonitrile, coagulation with ammonium acetate, and centrifugation made possible the direct analysis of plasma samples without the need for any further cleanup procedure. The developed method was validated with specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. All results were fully adequate. In the study using porcine plasma incurring enrofloxacin, the developed method proved capable of quantifying concentrations below its maximum residue limit, and its time-course residues were excreted within the 10-day withdrawal time. (C) 2011 Elsevier Ltd. All rights reserved.